| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.047204 |
| Chromosome: | chromosome 17 |
| Location: | 3649533 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g726526 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACACATTACTTTGTGACTATCCACCAAAA |
| Internal bar code: | GGCGTAAACTAAGGCTTACAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 177 |
| LEAP-Seq percent confirming: | 97.6331 |
| LEAP-Seq n confirming: | 2310 |
| LEAP-Seq n nonconfirming: | 56 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATACCCAGGACGGAAGTA |
| Suggested primer 2: | GCAAGGAAACAGAGAAACGC |