Insertion junction: LMJ.RY0402.047216_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.047216
Chromosome:chromosome 7
Location:1033904
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g319550 (1 of 1) PF10442 - FIST C domain (FIST_C) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCACGAGTGTGCAACACCCGCAAAGTTCT
Internal bar code:GACTCACTTATACGCCACCCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:197
LEAP-Seq percent confirming:99.2366
LEAP-Seq n confirming:260
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GGCATTAGGAGGACCAAACA
Suggested primer 2:AGCTTGCTGAGAGGTGTCGT