| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.047243 |
| Chromosome: | chromosome 5 |
| Location: | 1416756 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g244200 | TMA1,COTH2 | spore coat protein H; (1 of 3) PF08757 - CotH protein (CotH) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTGGGTGTGGGTGCTGAAAACTGCAATG |
| Internal bar code: | CGCCGCGGCCCCGTTGATTACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 208 |
| LEAP-Seq percent confirming: | 97.8218 |
| LEAP-Seq n confirming: | 2470 |
| LEAP-Seq n nonconfirming: | 55 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGACTTTACAAAGCCCCCT |
| Suggested primer 2: | ACAGCTGTTGTATGGGGAGG |