| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.047243 |
| Chromosome: | chromosome 7 |
| Location: | 3718386 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g337700 | PDC1 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCTGGACGGCGACAAGAACTTCTTCTTC |
| Internal bar code: | TATGATCGCCAGGCGGCGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 445 |
| LEAP-Seq percent confirming: | 98.2446 |
| LEAP-Seq n confirming: | 1623 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGCAGCTGGTGAGTTACTG |
| Suggested primer 2: | ACCAGCAATCCATCTTAGCG |