Insertion junction: LMJ.RY0402.047261_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.047261
Chromosome:chromosome 10
Location:663326
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre10.g422201 CTP4 Copper transporting ATPase; (1 of 2) K01533 - Cu2+-exporting ATPase (copB) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTCCTGGGGGGGGGGGGCGGTGTGGTGGG
Internal bar code:CCGCGCGTCGACGCAGTCGTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:225
LEAP-Seq percent confirming:96.3805
LEAP-Seq n confirming:2077
LEAP-Seq n nonconfirming:78
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:GGAAGGAGGGGCTTACACTC
Suggested primer 2:GGGGCGTCAATGATTGTTAT