| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.047283 |
| Chromosome: | chromosome 7 |
| Location: | 1811193 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325727 | (1 of 3) PTHR10566:SF76 - PROTEIN Y32H12A.7 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCAGATGTCCGACTGGTAACCCTGCGTCT |
| Internal bar code: | TTGCATTTGGCTTAGCACGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 694 |
| LEAP-Seq percent confirming: | 99.7819 |
| LEAP-Seq n confirming: | 2745 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTTGCTGGGTGTACAGGT |
| Suggested primer 2: | TGACATGGTTTGTTGCGTTT |