| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.047285 |
| Chromosome: | chromosome 3 |
| Location: | 5505070 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g185800 | (1 of 14) PF00892 - EamA-like transporter family (EamA) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGATGAGCATCACGCCCAGCATCATGAGGA |
| Internal bar code: | GCGGTCGCACACGCCAACGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 444 |
| LEAP-Seq percent confirming: | 99.5965 |
| LEAP-Seq n confirming: | 3949 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATTAGCCATCACCCAGACG |
| Suggested primer 2: | ACCACAGGATGTACTTCGCC |