| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.047289 |
| Chromosome: | chromosome 12 |
| Location: | 3963720 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g516500 | CUL4,CUL2 | (1 of 1) K10609 - cullin 4 (CUL4); Ubiquitin ligase SCF complex subunit Cullin | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGACAGCTTCGGCATGCACTGCCCGTACC |
| Internal bar code: | CTCAGTCGACCCGGGTCCGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 880 |
| LEAP-Seq percent confirming: | 99.651 |
| LEAP-Seq n confirming: | 7995 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTAGCCGTAGTTGTGCCAG |
| Suggested primer 2: | AACGTAAGGATGCAGGGATG |