Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.047320 |
Chromosome: | chromosome_3 |
Location: | 6722007 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g197750 | GPX3 | Glutathione peroxidase | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTTTGGACGCTTGGTCTTACCTGGGGGGTG |
Internal bar code: | CCGGGAGGCCTGTGCGCATATC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 753 |
LEAP-Seq percent confirming: | 98.8972 |
LEAP-Seq n confirming: | 8161 |
LEAP-Seq n nonconfirming: | 91 |
LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGACCACCCTTCTGGGTCTT |
Suggested primer 2: | CAGTCCCTGCAAGTGTCAGA |