| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.047324 |
| Chromosome: | chromosome 2 |
| Location: | 3693364 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095050 | (1 of 77) IPR012340 - Nucleic acid-binding, OB-fold | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGGGAGCTAAGGCGCGGCAACGGCAACT |
| Internal bar code: | ATAAGAAGAAGCAAACAAGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 458 |
| LEAP-Seq percent confirming: | 88.0282 |
| LEAP-Seq n confirming: | 125 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGAAGAAGCAAAACTCCG |
| Suggested primer 2: | GCCATGCCTCTTACGCTTAG |