| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.047347 |
| Chromosome: | chromosome 17 |
| Location: | 2140106 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g712700 | TNP45 | putative transposase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCTGAATTCAATGCACTGATGCTGATGG |
| Internal bar code: | GTGTGAGTGGGGCCGAAGCACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1239 |
| LEAP-Seq percent confirming: | 99.5378 |
| LEAP-Seq n confirming: | 10769 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACACTGACACGACACACCC |
| Suggested primer 2: | ACGCAGCAATCACTCTTCCT |