Insertion junction: LMJ.RY0402.047367_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.047367
Chromosome:chromosome 7
Location:4963917
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g346698 PHC75 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Putative pherophorin-chlamydomonas homolog intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGTGTGCTTGCTAACACACACACACACGC
Internal bar code:TCGTGACCCTGCATTCGTTACT

Confirmation - LEAP-Seq

LEAP-Seq distance:322
LEAP-Seq percent confirming:95.8495
LEAP-Seq n confirming:2471
LEAP-Seq n nonconfirming:107
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CGGTCTCCACATTTCTCGTT
Suggested primer 2:GCGTCCTAACTCACAGAGCC