| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.047370 |
| Chromosome: | chromosome 12 |
| Location: | 2395894 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g507250 | (1 of 1) K18681 - DIS3-like exonuclease 1 (DIS3L) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATGACGCTGACGGCGAATCGCTCGACGCC |
| Internal bar code: | TATTTCGATCTATTGTCCCACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 343 |
| LEAP-Seq percent confirming: | 98.4989 |
| LEAP-Seq n confirming: | 6562 |
| LEAP-Seq n nonconfirming: | 100 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACATACCGTGCATACCAG |
| Suggested primer 2: | GAATAGTCAGAAGGCGGCTG |