| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.047390 |
| Chromosome: | chromosome 16 |
| Location: | 4187237 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g668750 | MTA5 | Hypothetical protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCTCTTCCCTCCACGGGCCTTGACGCCA |
| Internal bar code: | GTGAGATGCCCCAAGGCACGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 476 |
| LEAP-Seq percent confirming: | 99.8211 |
| LEAP-Seq n confirming: | 558 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAAGGTGAGAGAGCCAAAG |
| Suggested primer 2: | AGTACGAGGCGCTACCGTAA |