| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.047418 |
| Chromosome: | chromosome 9 |
| Location: | 7292282 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g412750 | (1 of 1) K06228 - fused (FU) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCCGCGCCGCACGCTGCACGTGCAAAGT |
| Internal bar code: | GTCGGGTTGGATGGTTGGTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 497 |
| LEAP-Seq percent confirming: | 99.4483 |
| LEAP-Seq n confirming: | 42360 |
| LEAP-Seq n nonconfirming: | 235 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCCTCTCGTGCATCCTAC |
| Suggested primer 2: | AACTCAGCCTGCTCGGATTA |