| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.047426 |
| Chromosome: | chromosome 17 |
| Location: | 4841828 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g734100 | (1 of 1) 6.3.4.4 - Adenylosuccinate synthase / Succinoadenylic kinosynthetase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGAGGGGTACGCAGACGCGGGTGGGGCT |
| Internal bar code: | ATCGGCATCATCGCTTGCCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 88 |
| LEAP-Seq percent confirming: | 99.41 |
| LEAP-Seq n confirming: | 337 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAAATCGGTACCACCAAG |
| Suggested primer 2: | GAGCGAGTAAGGCACTGACC |