| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.047431 |
| Chromosome: | chromosome 9 |
| Location: | 508225 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g404100 | CLV3 | Voltage-gated chloride channel; (1 of 2) PTHR11689//PTHR11689:SF92 - CHLORIDE CHANNEL // H(+)/CL(-) EXCHANGE TRANSPORTER 7 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTACGGCGTGGGGAAGGTGATCTGCGAGGG |
| Internal bar code: | CGTTTTGGCTGCGAATTATATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 328 |
| LEAP-Seq percent confirming: | 96.0526 |
| LEAP-Seq n confirming: | 73 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCAACATCACCACGACCAC |
| Suggested primer 2: | AAGAATGAACGGGTGTGGAG |