Insertion junction: LMJ.RY0402.047431_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.047431
Chromosome:chromosome 9
Location:508244
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g404100 CLV3 Voltage-gated chloride channel; (1 of 2) PTHR11689//PTHR11689:SF92 - CHLORIDE CHANNEL // H(+)/CL(-) EXCHANGE TRANSPORTER 7 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCTGCTGGCTGTGGTGGGCGGCTACCTGG
Internal bar code:ACCCTAGACCTCGACTGTCGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:288
LEAP-Seq percent confirming:97.1608
LEAP-Seq n confirming:2635
LEAP-Seq n nonconfirming:77
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:CTCAACATCACCACGACCAC
Suggested primer 2:AAGAATGAACGGGTGTGGAG