Insertion junction: LMJ.RY0402.047460_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.047460
Chromosome:chromosome 7
Location:6083492
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g355433 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTTTACGAGCAACACATTGGCACATTGCT
Internal bar code:CATCGGCTATCTTGTGGAAGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:627
LEAP-Seq percent confirming:95.1437
LEAP-Seq n confirming:3311
LEAP-Seq n nonconfirming:169
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:TGCGCGCTAAACACTTATTG
Suggested primer 2:CTCCCACCTCAGATTGTGCT