| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.047462 |
| Chromosome: | chromosome 10 |
| Location: | 3256226 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g443050 | PFH3,PHX15,P4H3 | Prolyl 4-hydroxylase 3; (1 of 14) K00472 - prolyl 4-hydroxylase (E1.14.11.2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCCGTCGGCAACGGCGAGGGCCTGCAAG |
| Internal bar code: | TCGTGTGATACGGGATGCGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1167 |
| LEAP-Seq percent confirming: | 99.4865 |
| LEAP-Seq n confirming: | 775 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCATCTCAGCCTGCTCGTT |
| Suggested primer 2: | GAGGACATCCTTCCTGGTGA |