| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.047470 |
| Chromosome: | chromosome 3 |
| Location: | 9148160 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g212081 | (1 of 3) IPR013328 - 6-phosphogluconate dehydrogenase, domain 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGCTACTCCACCGCCGCCAAGAGCCGTA |
| Internal bar code: | AGCGCGTGCCCACGGCATAGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 981 |
| LEAP-Seq percent confirming: | 99.6883 |
| LEAP-Seq n confirming: | 5437 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCGCATTCATTCCTTTATT |
| Suggested primer 2: | GCTTTCGTCAGGTAAGCGTC |