| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.047484 |
| Chromosome: | chromosome 4 |
| Location: | 2753526 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g223600 | (1 of 1) IPR000104//IPR003072//IPR013328 - Antifreeze protein, type I // Orphan nuclear receptor, NOR1 type // 6-phosphogluconate dehydrogenase, domain 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGGACGTGGCAGTCGTGGTTGGATAGT |
| Internal bar code: | AAGATTGTTCTTCGGTGGGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 842 |
| LEAP-Seq percent confirming: | 98.7792 |
| LEAP-Seq n confirming: | 6311 |
| LEAP-Seq n nonconfirming: | 78 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGTGGTCACTGGGGAGTC |
| Suggested primer 2: | CACGCGCATATGGTTCATAC |