| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.047494 |
| Chromosome: | chromosome 10 |
| Location: | 1930239 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g432050 | FAP284 | Flagellar Associated Protein 284; (1 of 2) PF15239 - Domain of unknown function (DUF4586) (DUF4586) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGGGGTCGACGTACCGGTCACCCTGCCGG |
| Internal bar code: | GGCTCATCTTCCCCCTTCACCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1159 |
| LEAP-Seq percent confirming: | 99.7021 |
| LEAP-Seq n confirming: | 9705 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCAGCACTTTAGGCGATG |
| Suggested primer 2: | TGTAGATTTGCCGCAGTTTG |