| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.047540 |
| Chromosome: | chromosome 11 |
| Location: | 2006595 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g468374 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATACGGGTCAACTAGAAACAACATGGCGG |
| Internal bar code: | GATGTTTTGGGCCGACGGCGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1083 |
| LEAP-Seq percent confirming: | 99.3301 |
| LEAP-Seq n confirming: | 10380 |
| LEAP-Seq n nonconfirming: | 70 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACCACGTAGGCAGTGGAT |
| Suggested primer 2: | TGGAGAGGATTCCAGGACAC |