| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.047544 |
| Chromosome: | chromosome 7 |
| Location: | 3563596 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g336450 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGAGAACCCCGAGGCCAGCCGTGCCGCGT |
| Internal bar code: | TTGTTAGGCGATCTGCGCGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 453 |
| LEAP-Seq percent confirming: | 98.992 |
| LEAP-Seq n confirming: | 1866 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAAATGCTCGTTCCATTTT |
| Suggested primer 2: | GCACCTACGTACTTCGCACA |