| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.047568 |
| Chromosome: | chromosome 2 |
| Location: | 2171185 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g089550 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTAGGCAGGTATGCATGCACATCTGCTG |
| Internal bar code: | AACGCCGTATGTCTCATGTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 937 |
| LEAP-Seq percent confirming: | 99.5481 |
| LEAP-Seq n confirming: | 7049 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGAAGAAGCACCACAAGGC |
| Suggested primer 2: | CTGTATGTCCCCAAGGCTGT |