Insertion junction: LMJ.RY0402.047578_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.047578
Chromosome:chromosome_3
Location:4058937
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre03.g172550 PRMT1,PRM1 Protein-/Histone-arginine N-methyltransferase antisense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):TGAAATAGCATACACTCGCGGTCCGATGAA
Internal bar code:CGCTGTACGAGACTCCGCGTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:742
LEAP-Seq percent confirming:99.6127
LEAP-Seq n confirming:2829
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:CAATGAAGAACGTGGTGGTG
Suggested primer 2:AAGAACACCCAGCAATACCG