| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.047609 |
| Chromosome: | chromosome 13 |
| Location: | 4093188 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g591400 | COA1,COAA1 | (1 of 1) 2.7.1.33 - Pantothenate kinase / Pantothenic acid kinase; Pantothenate kinase, CoA biosynthesis | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCCGCCCCCACCCCCACCCGTCCATTCA |
| Internal bar code: | CGGGGTTCGGGTGGAGTGCGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 775 |
| LEAP-Seq percent confirming: | 99.7786 |
| LEAP-Seq n confirming: | 1803 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTACAGGACCAAGAGGGAA |
| Suggested primer 2: | CGTTAATGCCGTTTAGCCAT |