Insertion junction: LMJ.RY0402.047615_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.047615
Chromosome:chromosome 8
Location:3967003
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre08.g378750 CTAP7 Chloroplast translocon associated protein 7 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGGTGTCAACGATCAAATCCGCTTATGGC
Internal bar code:GTGATGGTCGTATGTACGCACG

Confirmation - LEAP-Seq

LEAP-Seq distance:518
LEAP-Seq percent confirming:99.1104
LEAP-Seq n confirming:10473
LEAP-Seq n nonconfirming:94
LEAP-Seq n unique pos:47

Suggested primers for confirmation by PCR

Suggested primer 1:GAAGTACCCAGCGAGTGAGC
Suggested primer 2:GTAGTATGCGGTGGCGTAGC