| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.047638 |
| Chromosome: | chromosome 11 |
| Location: | 2397431 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g474800 | OTA1 | Ornithine transaminase; (1 of 1) 2.6.1.13 - Ornithine aminotransferase / Ornithine--oxo-acid transaminase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGCAGTGAGATCTGGGCACCGCAGCCGC |
| Internal bar code: | GACGTTAGGTCCGGCTATTTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 133 |
| LEAP-Seq percent confirming: | 97.9592 |
| LEAP-Seq n confirming: | 144 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGTCCCACAGAAACACAC |
| Suggested primer 2: | GCTAGCGCACTTACCCTGTC |