| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.047656 |
| Chromosome: | chromosome 9 |
| Location: | 2207850 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g392950 | TMG12 | tRNA (guanosine-2'-O)-methyltransferase; (1 of 1) PTHR12029//PTHR12029:SF55 - RNA METHYLTRANSFERASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCTGCAGTCGGGCGGCCGCGTGTGATAG |
| Internal bar code: | AGCTAGCGAAACGCCGGTCGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 126 |
| LEAP-Seq percent confirming: | 92.1951 |
| LEAP-Seq n confirming: | 378 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGCGCTCTACATCTCCTTT |
| Suggested primer 2: | CTGGAAGCGTAGGGACAGAG |