| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.047688 |
| Chromosome: | chromosome 9 |
| Location: | 4216482 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g394325 | ELI3 | Early light-inducible protein | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAATCGAAGTACGACTGGCCTCAATCGTGT |
| Internal bar code: | CGTCCCTCTCGGACGACGCCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1428 |
| LEAP-Seq percent confirming: | 99.3704 |
| LEAP-Seq n confirming: | 947 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTAAGGCAGGTTGGTGAA |
| Suggested primer 2: | AAGCGCTAACCAAACGCTAA |