| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.047689 |
| Chromosome: | chromosome 12 |
| Location: | 2691560 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g503850 | ARLP2,ARL12 | ARF-like GTPase; (1 of 4) K07937 - ADP-ribosylation factor 1 (ARF1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGCAGAAGGGGAAAGGTAGGGTCGACCA |
| Internal bar code: | GGACGGGTCTGCGCCGTTTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 943 |
| LEAP-Seq percent confirming: | 96.2347 |
| LEAP-Seq n confirming: | 1968 |
| LEAP-Seq n nonconfirming: | 77 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACCTCATCAATCGTGCAT |
| Suggested primer 2: | GTGGTAGGTGGTGCCAGTCT |