| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.047691 |
| Chromosome: | chromosome 14 |
| Location: | 3331166 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGGGAGCGGCCAGGATGGGGAGAGCTGA |
| Internal bar code: | CATAATTTTATCGTTATTGAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1033 |
| LEAP-Seq percent confirming: | 96.3504 |
| LEAP-Seq n confirming: | 396 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATTGATTAGCACAGCGCAA |
| Suggested primer 2: | TTAGGTGTGTGGGTCAACGA |