Insertion junction: LMJ.RY0402.047696_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.047696
Chromosome:chromosome 9
Location:1950877
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g394900 CYA10 (1 of 1) IPR000209//IPR001054//IPR029787 - Peptidase S8/S53 domain // Adenylyl cyclase class-3/4/guanylyl cyclase // Nucleotide cyclase; Solute-binding protein-like adenylate cyclase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATGTATGGTTGTTGGTTGGGCGTGGTTCT
Internal bar code:TCGTTGGGGGTGCAGCGGGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:986
LEAP-Seq percent confirming:98.4902
LEAP-Seq n confirming:7567
LEAP-Seq n nonconfirming:116
LEAP-Seq n unique pos:33

Suggested primers for confirmation by PCR

Suggested primer 1:TTTGCTGATGAGTCGGTCAC
Suggested primer 2:TGTTGCGTGTGATAACGGAT