Insertion junction: LMJ.RY0402.047699_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.047699
Chromosome:chromosome 2
Location:3392887
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g095103 GT90-34,GT90F34 GT90 family protein 34; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCAATATGTGTGCGGCAGTGTGAACATCC
Internal bar code:CTCGGTCTGGTAAACGCACTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:980
LEAP-Seq percent confirming:99.4411
LEAP-Seq n confirming:15123
LEAP-Seq n nonconfirming:85
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:CTGTTCGCTGTGGAAAGACA
Suggested primer 2:TAGCAGTGACAGCGGAAATG