| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.047706 |
| Chromosome: | chromosome 7 |
| Location: | 3500091 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g335900 | CPZ | (1 of 1) 3.4.17.3 - Lysine carboxypeptidase / Lysine(arginine) carboxypeptidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGCGTGCTATTGCTATGTGCAAAGTATA |
| Internal bar code: | TTATATTCTTGCCAGTAGTAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 700 |
| LEAP-Seq percent confirming: | 99.267 |
| LEAP-Seq n confirming: | 948 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCACTGACCTTGGCTACC |
| Suggested primer 2: | ACCCAAGCGTACATTTCCAG |