| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.047714 |
| Chromosome: | chromosome 1 |
| Location: | 4337954 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g029400 | (1 of 6) PTHR31942//PTHR31942:SF1 - FAMILY NOT NAMED // MLO-LIKE PROTEIN 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGCGACGGCGGGAACGGCCCCGGGTAGCA |
| Internal bar code: | CAAGGACGGCTGAGGTCTGAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 533 |
| LEAP-Seq percent confirming: | 99.8141 |
| LEAP-Seq n confirming: | 1074 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCAGATGAGGGCTGAGGAG |
| Suggested primer 2: | CCAGCCTAGCACTATGCCTC |