| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.047716 |
| Chromosome: | chromosome_9 |
| Location: | 3647698 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g390430 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GACTTGGAGGAGAGGGGCCGCTGCAGGAGC |
| Internal bar code: | GATTCCCCGGCCCTTATGCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 515 |
| LEAP-Seq percent confirming: | 82.2884 |
| LEAP-Seq n confirming: | 10644 |
| LEAP-Seq n nonconfirming: | 2291 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCAGTCTTCACGCAAGTC |
| Suggested primer 2: | GATATGCGGATACGGGTTTG |