| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.047726 |
| Chromosome: | chromosome 3 |
| Location: | 7351200 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g206500 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCCCCCGTCCCTACCTGCCGGTGCAGCG |
| Internal bar code: | GTCTTGTCTCTATCCTCGCTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 419 |
| LEAP-Seq percent confirming: | 90.0713 |
| LEAP-Seq n confirming: | 13771 |
| LEAP-Seq n nonconfirming: | 1518 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGATTGACACGGACAGGC |
| Suggested primer 2: | ATCCCCTCCACCTCTGTCTT |