Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.047746 |
Chromosome: | chromosome 12 |
Location: | 4708975 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre12.g523550 | UMM11 | UbiE/COQ5 methyltransferase family protein; (1 of 1) PTHR10108//PTHR10108:SF752 - METHYLTRANSFERASE // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCACGGCACCACGCCCTTGTTCACTGCC |
Internal bar code: | CGTTGCTACTAAAGTAAAGAGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 891 |
LEAP-Seq percent confirming: | 99.7123 |
LEAP-Seq n confirming: | 10746 |
LEAP-Seq n nonconfirming: | 31 |
LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CACCTTCACTCTGCTCCTCC |
Suggested primer 2: | GGACTGATAGCCCCGTTACA |