Insertion junction: LMJ.RY0402.047746_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.047746
Chromosome:chromosome 12
Location:4708975
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g523550 UMM11 UbiE/COQ5 methyltransferase family protein; (1 of 1) PTHR10108//PTHR10108:SF752 - METHYLTRANSFERASE // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCCACGGCACCACGCCCTTGTTCACTGCC
Internal bar code:CGTTGCTACTAAAGTAAAGAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:891
LEAP-Seq percent confirming:99.7123
LEAP-Seq n confirming:10746
LEAP-Seq n nonconfirming:31
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:CACCTTCACTCTGCTCCTCC
Suggested primer 2:GGACTGATAGCCCCGTTACA