| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.047760 |
| Chromosome: | chromosome 1 |
| Location: | 1364582 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g006800 | GPI1,PIGA1,SETH2,GTR18 | Phosphatidylinositol N-acetylglucosaminyltransferase, catalytic subunit; (1 of 1) K03857 - phosphatidylinositol glycan, class A (PIGA, GPI3) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGACGCGGGCCATGCCGCCTTTGCTAAGC |
| Internal bar code: | TGCCGCAGGTCGACACGATGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 640 |
| LEAP-Seq percent confirming: | 99.6201 |
| LEAP-Seq n confirming: | 2098 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTTTTGTTGGGTGGGTAGC |
| Suggested primer 2: | GAACCCTAAGTCACCCGACA |