| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.047766 |
| Chromosome: | chromosome 1 |
| Location: | 2174396 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g011901 | ARS7 | Arylsulfatase; (1 of 19) 3.1.6.1 - Arylsulfatase / Sulfatase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCCCGACGACTGCGCCGAGCGCCAGGGA |
| Internal bar code: | CATCGGGCGGCCGTGAGCATAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 460 |
| LEAP-Seq percent confirming: | 96.7941 |
| LEAP-Seq n confirming: | 3925 |
| LEAP-Seq n nonconfirming: | 130 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATTGCCGTCAACACAAATG |
| Suggested primer 2: | GCAACCTGTGGGCTACAAAT |