Insertion junction: LMJ.RY0402.047783_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.047783
Chromosome:chromosome 6
Location:5246946
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g282300 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACTCGCCGCTCCCACTGAATCTGATGACC
Internal bar code:TCTGGCGATTTTGTTGCGATTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1160
LEAP-Seq percent confirming:99.4939
LEAP-Seq n confirming:12778
LEAP-Seq n nonconfirming:65
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:TGGTGGTACATTGGAGGGTT
Suggested primer 2:CCCACGAGTCTCAGGAAGAG