| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.047788 |
| Chromosome: | chromosome 3 |
| Location: | 1225114 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g149700 | (1 of 1) PF14518 - Iron-containing redox enzyme (Haem_oxygenas_2) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGCAAGTACGCACACGGGCCGCTGGGTC |
| Internal bar code: | GACCAGGTTGTCTGTGGTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 201 |
| LEAP-Seq percent confirming: | 99.7704 |
| LEAP-Seq n confirming: | 1738 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCAATTTGCGCTCTGTGTC |
| Suggested primer 2: | TGTAAGCACTCACTGGAGCG |