Insertion junction: LMJ.RY0402.047806_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.047806
Chromosome:chromosome 2
Location:6397717
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g115508 (1 of 1) IPR001611//IPR003590//IPR003591 - Leucine-rich repeat // Leucine-rich repeat, ribonuclease inhibitor subtype // Leucine-rich repeat, typical subtype 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCGAATCCCTCACGGCACCACCTGAATGC
Internal bar code:GCAAATGTGCCCTATGTTTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:869
LEAP-Seq percent confirming:97.0372
LEAP-Seq n confirming:5666
LEAP-Seq n nonconfirming:173
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GGGTACGTGTTGTGCAACTG
Suggested primer 2:GGAGGCACCATCCTCATAGA