| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.047826 |
| Chromosome: | chromosome 13 |
| Location: | 1885358 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g575750 | (1 of 1) 2.5.1.105 - 7,8-dihydropterin-6-yl-methyl-4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate synthase / Dihydropteroate synthase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTTTCGTTCCCTTCTGCTCACTAACACA |
| Internal bar code: | AGAACACCCCCCGACTACGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 671 |
| LEAP-Seq percent confirming: | 89.1775 |
| LEAP-Seq n confirming: | 412 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTACCTGCCTCCAGCATTC |
| Suggested primer 2: | ACACGTAGAACAGCCCATCC |