| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.047829 |
| Chromosome: | chromosome 9 |
| Location: | 7864447 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g416900 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTACCATTACCACTGCCTCAACCCGAACGT |
| Internal bar code: | CGTTATGGAACCCTTGCGAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 799 |
| LEAP-Seq percent confirming: | 98.0337 |
| LEAP-Seq n confirming: | 7678 |
| LEAP-Seq n nonconfirming: | 154 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAAACAAACGGTCTCCAGT |
| Suggested primer 2: | GCGCCAACAGTCACTCTGTA |