| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.047853 |
| Chromosome: | chromosome 7 |
| Location: | 3388753 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g335150 | (1 of 1) IPR004333//IPR019831 - Transcription factor, SBP-box // Manganese/iron superoxide dismutase, N-terminal | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGAGGTGTTGGTGTGCTGTAGTCGTGGC |
| Internal bar code: | GCTTTAACTGGCTTCTAATGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1176 |
| LEAP-Seq percent confirming: | 99.6232 |
| LEAP-Seq n confirming: | 5023 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGAGGTTATTGGAGCGTT |
| Suggested primer 2: | ACTTGTTGGAAGACCCGTTG |