| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.047904 |
| Chromosome: | chromosome 6 |
| Location: | 6065023 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g289600 | (1 of 781) IPR000104 - Antifreeze protein, type I | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTGGCACGTTCAGGAAGGCGGTATCGAT |
| Internal bar code: | TCACTGCGCGGTTCGGCGCAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 474 |
| LEAP-Seq percent confirming: | 99.686 |
| LEAP-Seq n confirming: | 1270 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGGAGTTCAGGAACAGGT |
| Suggested primer 2: | AGGCGTTAATGGTGTCTTGG |